Although not all of these side effects may occur, if they do occur they may need medical attention. Check with your doctor immediately if any of the following side effects occur while taking chloroquine: Incidence not known Some side effects of chloroquine may occur that usually do not need medical attention. Side effects stopping plaquenil What is retinopathy hydroxychloroquine Jul 30, 2019 Before the administration of chloroquine, the patient had only a mild skin erythema in the irradiated area, which was consistent with the radiotherapy dose she had received. On day 3 of chloroquine therapy, she developed localized brisk bullous eruptions in the irradiated area, which developed into a patch of fulminant moist desquamation. Calcium phosphate co-precipitation as a transfection method was introduced in the early 1970s Graham and van der Eb, 1973. The method is still popular because the components are easily available and inexpensive, the protocol is easy to use, and it is effective with many different types of cultured cells. The calcium phosphate transfection method for introducing DNA into mammalian cells is based on forming a calcium phosphate-DNA precipitate. Calcium phosphate facilitates the binding of the DNA to the cell surface. DNA then enters the cell by endocytosis. The method was first developed by Graham and van der Ebb and was later modified by Wigler. Also, your health care professional may be able to tell you about ways to prevent or reduce some of these side effects. These side effects may go away during treatment as your body adjusts to the medicine. Chloroquine calcium phosphate transfection High efficiency polyoma DNA transfection of chloroquine., Transfection Guide Overview of Transfection Methods Promega Cardiomyopathy caused by plaquenilFiller used in hydroxychloroquinePlaquenil treatment lyme disease Calcium phosphate transfection is a commonly used method for the introduction of DNA into eukaryotic cells. This technique has been used to obtain both transient 1 and stable 2 transfections in a wide variety of cell types. The procedure is based on slow mixing of HEPES-buffered saline containing sodium phosphate with a CaCl 2 solution. Calcium Phosphate Transfection Kit Protocol Sigma-Aldrich. Calcium Phosphate Transfection Kit. Aralen Chloroquine Uses, Dosage, Side Effects.. Calcium Phosphate Transfection of 293T cells 2 Transfection 1. Twenty-four hours prior to transfection, inoculate 1 to 2 x106 cells/10 cm plate or, inoculate 3x106 cells/plate 16-hours prior to transfection. in 10 ml DMEM media + 10% BCS supplemented with L-glutamine and Pen/Strep. At the time of transfection, cells should be about 30-40%. Plate cells the night before to give 60-80% confluence at the day of transfection. 2. One hour prior to the transfection, change to medium containing 25µM chloroquine from x1000 stock in PBS, stored at -20°C. Volume should be 10 ml per dish. Chloroquine can be omitted, but increases efficiency about x2 3. Calcium phosphate transfection is one of the most commonly used methods to deliver plasmid DNA into mammalian cells. Due to its highly consistent pH and salt concentration, our CalPhos calcium phosphate transfection kit gives you the consistent transfection efficiency that you cannot always achieve with home brew versions.